A one - headed class V myosin molecule develops multiple large ( 32 - nm ) steps successively
نویسندگان
چکیده
*Formation of Soft Nanomachines, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, †Single Molecule Processes Project, International Cooperative Research Project, Japan Science and Technology Agency, ‡Department of Biophysical Engineering, Osaka University, §Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, and ¶Dynamic Nanomachine Project, International Cooperative Research Project, Japan Science and Technology Agency, 1-3, Yamadaoka, Suita, Osaka 565-0871, Japan; and Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655-0127
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Mechanical Characterization of One-Headed Myosin-V Using Optical Tweezers
Class V myosin (myosin-V) is a cargo transporter that moves along an actin filament with large (approximately 36-nm) successive steps. It consists of two heads that each includes a motor domain and a long (23 nm) neck domain. One of the more popular models describing these steps, the hand-over-hand model, assumes the two-headed structure is imperative. However, we previously succeeded in observ...
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It is widely accepted that the vesicle-transporter myosin-V moves processively along F-actin with large steps of approximately 36 nm using a hand-over-hand mechanism. A key question is how does the rear head of two-headed myosin-V search for the forward actin target in the forward direction. Scanning probe nanometry was used to resolve this underlying search process, which was made possible by ...
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